Composite

Part:BBa_K3196011:Design

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)


AOX1-Kozak-FLO10-SLAC-His tag-AOX1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1387


Design Notes

Source

References

[1] T. Kudanga, M. Le Roes-Hill, Laccase applications in biofuels production: current status and future prospects, Appl. Microbiol. Biotechnol., 98 (2014), pp. 6525-6542;

[2] D.M. Mate, M. Alcalde, Laccase engineering: from rational design to directed evolution, Biotechnol. Adv., 33 (2015), pp. 25-40;

[3]D. Yadav, B. Ranjan, N. Mchunu, M.L. Roes-Hill, T. Kudanga. Secretory expression of recombinant small laccase from Streptomyces coelicolor A3(2) in Pichia pastoris, Int. J. Biol. Macromol., 108 (2018), pp. 642-649;